Tag

Part:BBa_K390004:Design

Designed by: Cody Tramp   Group: iGEM10_Utah_State   (2010-08-01)

art+ secretion tag for Synechocystis sp. PCC 6803


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part must be used with an assembly standard that supports fusion proteins, or the scar site will need to be modified so that there is no frame shift in the downstream gene.

Source

This part is based on a sequence that was artificially designed in Sergeyenko and Los, 2003. It was created by annealing two oligionucleotides together.

References